Tag: ffpe samples

How To Extract Dna From FFPE Tissue Samples?

DNA extraction from FFPE tissue samples is a common practice in forensics. There are a few different methods that can be used to extract DNA, and the one that is chosen will depend on the type of sample and the particular equipment that is available. If you are looking to buy FFPE tissue samples for DNA research, visit https://www.geneticistinc.com/blog/ffpe-samples.

 

The most common method of DNA extraction is called phenol-chloroform extraction. This involves soaking the sample in a solution of phenol and chloroform, then extracting the DNA using a solvent such as ethanol. This technique is simple and reliable, but it can be time-consuming and it can be difficult to get good results from samples that contain high levels of moisture or fat.

Another method that is sometimes used for DNA extraction is called enzymatic digestion. This technique involves breaking down the cell walls of the sample using an enzyme, then removing the DNA using a solvent such as an ethyl acetate. This method is faster than phenol-chloroform extraction, but it can be less effective at removing small amounts of DNA from samples.

If there is no suitable method for extracting DNA from the sample, then mitochondrial PCR may be necessary. This technique uses genetically modified microorganisms to copy pieces of mitochondrial DNA into new colonies of bacteria. After the DNA has been copied, it can be isolated and analyzed.

DNA extraction is a common practice in forensics, and there are a variety of different methods that can be used to extract DNA from samples. The method that is chosen will depend on the type of sample and the particular equipment that is available.